Asparginase activity of some earthworm-borne bacteria and actinomycetes was assayed. Though all the bacteria understudy secreted asparaginase, the degree of production of asparaginase varied with the organism. Micrococcus roseus and Rhodococus sp. secreted maximum L-asparaginase.Where as Flavimonas orhyzihabitans,Xanthomonas maltophilia was found to be poor in the secret of L-asaparaginase. M. roseus, Rhodococus sp, B.mycoides and B.macerans opted for D.mannose and mannitol; lactose and sorbose ; maltose and starch ; galactose respectively nitrogen source differed among the bacteria under study.