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A four nanoprobe system has been installed inside a FEI XL30 F scanning electron microscope (SEM), and shown to be fully compatible with the normal functions of the SEM and also a Gatan cold stage (model C1003, 2 185-400 8C). With some selected examples of applications, we have shown that this nanoprobe system may be used effectively for gripping, moving and manipulating nanoobjects, e.g. carbon nanotubes, setting up electric contacts for electronic measurements, tailoring the structure of the nanoobject by cutting, etc. and even for making unexpected nanostructures, e.g. a nanohook. Applications in other areas have also been speculated, limitations or disadvantages of the current design of the probe system were discussed, and methods for possible improvement were suggested. q

KEYWORDSCryo-electron tomography, cryo-focused ion beam, lift-out, high-pressure freezing, in situ structural biology 3 Abstract Cryo-focused ion beam milling of frozen hydrated cells for the production of thin lamellas in combination with cryo-electron tomography (cryo-ET) has yielded unprecedented insights into the cell interior. This method allows access to native structures deep inside cells, enabling structural studies of macromolecules in situ. However, it is only suitable for cells that can be vitrified by plunge freezing (<10 μm). Multicellular organisms and tissues are considerably thicker and high-pressure freezing is required to ensure optimal preservation. Here, we describe a preparation method for extracting lamellas from high pressure frozen samples with a new cryo-gripper tool. This in situ lift-out technique at cryo-temperatures enables cryo-ET to be performed on multicellular organisms and tissue, extending the range of applications for in situ structural biology.

Articles you may be interested inA novel flexure-based microgripper with double amplification mechanisms for micro/nano manipulation Rev. Sci. Instrum. 84, 085002 (2013); 10.1063/1.4817695 Design and experimental research of a novel inchworm type piezo-driven rotary actuator with the changeable clamping radius Rev. Sci. Instrum. 84, 015006 (2013); 10.1063/1.4788736Particle manipulation using an ultrasonic micro-gripper Appl.A novel driving principle by means of the parasitic motion of the microgripper and its preliminary application in the design of the linear actuator Rev. Sci. Instrum. 83, 055002 (2012);Since the manipulation of biological objects is usually performed in a life-sustaining environment, electrical fields or thermal gradients through the liquid may cause perturbations. The authors present a microgripper fabricated in silicon by a combination of bulk and surface micromachining processes that exhibits several advantages compared to previous reports. In order to avoid any possible perturbation caused by electrical fields, their microgripper is mechanically actuated. The complete system including the microgripper, a piezoactuator, and a nanomanipulator is described in detail together with manipulation of micrometer sized glass spheres.

In recent years, nanoscale fabrication has developed considerably, but the fabrication of free-standing nanosize components is still a great challenge. The fabrication of metallic nanocomponents utilizing three basic steps is demonstrated here. First, metallic alloys are used as factories to produce a metallic raw stock of nano-objects/nanoparticles in large numbers. These objects are then isolated from the powder containing thousands of such objects inside a scanning electron microscope using manipulators, and placed on a micro-anvil or a die. Finally, the shape of the individual nano-object is changed by nanoforging using a microhammer. In this way free-standing, high-strength, metallic nano-objects may be shaped into components with dimensions in the 100 nm range. By assembling such nanocomponents, high-performance microsystems can be fabricated, which are truly in the micrometre scale (the size ratio of a system to its component is typically 10:1). M This article features online multimedia enhancements