Wood material from the sapwood of the studied tree species is as useful as cellulose for studying environmental effects on tree-ring δ(18)O and δ(13)C values at a short-term scale as considered in most ecophysiological studies. The more variable response of oak may require further investigations.
Summary
The wheat
Lr34res
allele, coding for an
ATP
‐binding cassette transporter, confers durable resistance against multiple fungal pathogens. The
Lr34sus
allele, differing from
Lr34res
by two critical nucleotide polymorphisms, is found in susceptible wheat cultivars.
Lr34res
is functionally transferrable as a transgene into all major cereals, including rice, barley, maize, and sorghum.
Here, we used transcriptomics, physiology, genetics, and
in vitro
and
in vivo
transport assays to study the molecular function of
Lr34
.
We report that
Lr34res
results in a constitutive induction of transcripts reminiscent of an abscisic acid (
ABA
)‐regulated response in transgenic rice.
Lr34‐
expressing rice was altered in biological processes that are controlled by this phytohormone, including dehydration tolerance, transpiration and seedling growth.
In planta
seedling and
in vitro
yeast accumulation assays revealed that both
LR
34res and
LR
34sus act as
ABA
transporters. However, whereas the
LR
34res protein was detected
in planta
the
LR
34sus version was not, suggesting a post‐transcriptional regulatory mechanism.
Our results identify
ABA
as a substrate of the
LR
34
ABC
transporter. We conclude that
LR
34res‐mediated
ABA
redistribution has a major effect on the transcriptional response and physiology of
Lr34res
‐expressing plants and that
ABA
is a candidate molecule that contributes to
Lr34res
‐mediated disease resistance.
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