<p>Introduction: Breast cancer can be classified into luminal A, luminal B, Her2+, dan basal-like molecular subtype according to genetic profile and immunophenotype. The aim of this study is to assess histopathologic and immunophenotype characteristics of breast cancer in Cipto Mangunkusumo Hospital Jakarta, Indonesia.<br />Material and methods: This study is a cross-sectional retrospective study. The sample was 2632 breast cancer case in Department of Anatomical Pathology Cipto Mangunkusumo Hospital from the year 2011 until 2015. ER, PR, Her2, Top2 α, and Ki67 immunohistochemical stain was then grouped into luminal A (ER+/PR+/Her2-/Ki67&lt;20%) subtype, luminal B (ER+/PR+/Her2-/Ki67≥20%) subtype, Her2+ (ER-/PR-/Her2+) subtype, and basal-like (ER-/PR-/Her2-) subtype. Kendall’s Tau analysis was done to assess association between sample characteristics and molecular subtype, association between top2α positive and molecular subtype.<br />Result: The most prevalent to the less prevalent breast cancer molecular subtype was luminal B (43,9%), Her2+ (14,6%), luminal A (14,0%), and basal-like (11,3%). There was a significant association between the tumor stage and breast cancer subtype (p=0,0001). There is also a significant association between Top2α expression and molecular subtype (p=0,0001).<br />Conclusion: The most prevalent breast cancer molecular subtype was luminal B. There was an association between breast cancer molecular subtype and a higher degree and Top2α expression.</p>

Xylol adalah agen deparafinisasi yang umum digunakan ditiap tahapan deparafinisasi pada pewarnaan histologi jaringan. Namun karena sifat toksisitas tinggi dan harga yang mahal, maka diupayakan mencari alternatif yang lebih aman dan murah. Ez Prep concentrate adalah salah satu agen deparafinisasi yang umum digunakan pada alat Ventana Medical Systems, Inc (Ventana). Produk ini dirancang untuk digunakan sebagai pewarna slide otomatis BenchMark Series. Selain harga nya lebih murah dibanding xylol, toksisitasnya juga rendah. Perlu dibuktikan efektifitas reagen EZ Prep concentrate agar dapat digunakan sebagai alternatif pengganti xylol. Penelitian ini bersifat eksperimen yang dilaksanakan pada bulan September – November 2022 di Laboratorium Patologi Anatomi Siloam Hospital Lippo Village. Sampel yang digunakan jaringan miom dan payudara. Hasil penelitian menunjukkan semua sampel yang dideparfinasi dengan EZ prep concentrate memberikan kualitas hasil mikroskopis yang baik (skor 3). Maka, EZ Prep concentrate dapat dipertimbangakan penggunaannya sebagai pengganti xylol.

<p>Peritoneal Malignant Mesothelioma (PMM) is uncommon disease, but increasing in frequency nowadays. This highly aggressive malignancy occurs most commonly in older men and has a strong association with asbestos exposure. It manifests most often as diffuse sheet-like or nodular thickening of the peritoneal surfaces, but it may occasionally be a localized mass. The very large variations of its clinical features and its histological appearance mimicking adenocarcinoma make this tumor is difficult to diagnose.<br />We report a case of PMM that previously diagnose as adenocarcinoma of the ovary. A 29 year-old female came to gynecology clinic with para-ovarian mass. She had no history of asbestos exposure. The mass was oval 9x6x6 cm in size, whitish and firm. Microscopic features showed papillary dense structure with desmoplastic stroma, covered by a layer of cuboidal to columnar cells. The cells with mild pleomorphism and hyperchromatic nuclei, mitotic figures were minimal. The immunohistochemistry tests revealed positive for D2-40, Calretinin, CK8 and CK 18, weakly positive for Inhibin and EMA, and negative for CEA and AFP. Patient had been received chemotherapy, there were no metastasis.</p>

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