Entomopathogenic nematodes (EPN) are one of the soil worms that have been widely used as a natural pest control. EPN has its pathogenic capability because of the mutualistic interactions between nematodes and symbiotic bacteria inside the digestive tract of nematodes. Symbiotic bacteria capable of producing exoenzymes that are toxic to insects. The isolation of symbiotic bacteria accomplished by infection of obtained EPN (Belik II isolate) into Tenebrio molitor larvae. Symbiotic bacteria were isolated from the hemolymph of dead larvae on NBTA media. Isolation of symbiotic bacteria was successfully obtained two morphologically distinct bacteria: B 3.1 isolate and B 4 isolate. Both bacteria were further identified using PCR analysis of the 16S rRNA gene. Based on the sequencing results, the B 3.1 isolate was in accordance with Acinetobacter pittii strain ATCC 19004, while B 4 isolate was in accordance with bacteria Enterobacter aerogenes strain KCTC 2190. The characterization of B 3.1 isolate was shown to have similarities with Acinetobacter sp., i.e.: gram-negative, non - motile, rod-shaped, and some other characteristics of biochemical tests. While the characterization of B 4 isolate was shown to have similarities with E. aerogenes i.e.: gram-negative, rod-shaped, motile, and some other characteristics of biochemical tests. These findings will be the potential to be applied as biological agents in pest control.

Artikel ini membahas mengenai analisis form, konten, dan konteks dari logo Badan Kependudukan dan Keluarga Berencana Nasional (BKKBN) yang dilakukan pada tahun 2020. Analisis ini dilakukan dalam lingkup penelitian tindakan kelas yang dilakukan oleh penulis, khususnya siklus ketiga. Dalam siklus ketiga ini, penulis melakukan analisis visual dengan menggunakan metode analisis form, konten, dan konteks yang telah dikembangkan sebelumnya. Berdasarkan analisis yang dilakukan, dapat dipahami nilai lebih dan kekurangan dari logo baru BKKBN: dimana logo mungkin dapat dirancang dengan baik secara formal, namun memiliki beberapa kekurangan dalam kemampuan representasi logo tersebut.