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Complexing of 3,4-benzpyrene (BP) with a series of nucleosides, 5 '-nucleotides, native and denatured deoxyribonucleic acid (DNA), calf thymus nucleohistone, synthetic polyribonucleotides, and yeast ribonucleic acid (RNA) has been investigated using radiochemical assay methods. Physical binding to the polynucleotides and nucleohistone has been demonstrated by sucrose gradient electrophoresis and ultracentrifugation.

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Increasing number of CCC/sample correlates with the adverse outcome and poorer survival (P < 0.0001). Our CCC test based on the negative selection procedure may provide valuable prognostic information.