The reaction ' C(t,p) has been used to locate 1p-2h and 2p-3h states in ' C. Results for the negative-parity levels are in good agreement with expectations for (sd) L =0, 2, and 4 transfers as given by ' C(t,p)' C. NUCLEAR REACTIONS ' C(t,p)' C; E = 18 MeV; measured cr(Ep, 0), 8=3.75 -86. 25' (lab) ' C levels, L, J, comparison with ' C.

The PI3K/AKT/mTOR (PAM) signaling pathway is frequently mutated in prostate cancer. Specific AKT inhibitors are now in advanced clinical trials, and this study investigates the effect of MK2206, a non-ATP-competitive inhibitor, on the cellular metabolism of prostate cancer cells. We observed a reduction in cell motility and aerobic glycolysis in prostate cancer cells with treatment. These changes were not accompanied by a reduction in the ratio of high-energy phosphates or a change in total protein levels of enzymes and transporters involved in glycolysis. However, a decreased ratio of NAD/NADH was observed, motivating the use of hyperpolarized magnetic resonance spectroscopy (HP-MRS) to detect treatment response. Spectroscopic experiments were performed on tumor spheroids, 3D structures that self-organize in the presence of an extracellular matrix. Treated spheroids showed decreased lactate production with on-target inhibition confirmed using IHC, demonstrating that HP-MRS can be used to probe treatment response in prostate cancer spheroids and can provide a biomarker for treatment response. .

Hyperpolarized magnetic resonance spectroscopy (HP MRS) using dynamic nuclear polarization (DNP) is a technique that has greatly enhanced the sensitivity of detecting 13C nuclei. However, the HP MRS polarization decays in the liquid state according to the spin-lattice relaxation time (T1) of the nucleus. Sampling of the signal also destroys polarization, resulting in a limited temporal ability to observe biologically interesting reactions. In this study, we demonstrate that sampling hyperpolarized signals using a permanent magnet at 1 Tesla (1T) is a simple and cost-effective method to increase T1s without sacrificing signal-to-noise. Biologically-relevant information may be obtained with a permanent magnet using enzyme solutions and in whole cells. Of significance, our findings indicate that changes in pyruvate metabolism can also be quantified in a xenograft model at this field strength.

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The stereoselective synthesis of E-and Z-isomers of a Cmannosyl crotylpinacolboronate via Ni-promoted reactions on an allylic acetate and a diene precursor, respectively, is described. The E-and Zisomers reacted with 1,2-O-isopropylidene glyceraldehyde in the presence or absence of (R)-and (S)-TRIP catalysts, to give predominantly 3,4-anti and 3,4-syn crotylation products, respectively, with moderate to high facial selectivity. These products were transformed to biologically relevant C-manno-disaccharides.