Coccidiosis has a major impact on poultry industry as it affects broiler and layer birds of all age groups. Caecal coccidiosis, caused by is a very devastating enteric disease in broiler,which involves huge economic loss experimental infective dose of in broiler th th chicken and subsequent alterations in different plasma biochemical constituents were evaluated at interval of 5 , 7 and th 9 day of post inoculation (PI) with the selected dose of 20000-25000 sporulated oocyst per bird. The dose was selected based on titration. A significant increase in plasma glucose, total cholesterol level and aspartate aminotransferase (AST) activity were observed where as a significant decrease in the level of total plasma protein, albumin, globulin, triglyceride and alanine aminotransferase (ALT) activity were evident during infection

The present study investigate the effects of oral administration of ayurvedic nanoparticle drug Rasamanikya against ESBL producing gastrointestinal gram-negative bacteria Escherichia coli in experimental broiler chicken and haemato-biochemical, pathological alteration due to drug administration. At the age of 21 days, the birds were divided into three groups i.e. group-C served as control group, group-E treated with Enrofloxacin which act as standard control group and group-RM treated with test drug Rasamanikya, containing six birds in each group. On the day 22, the experiment (Drug trial) was started and continued for next 28 days. On the day 0, 7, 14, 21 and 28 of experiment fecal sample was collected by using fecal swab from each bird of three groups to examine the ESBL producing gastrointestinal gram-negative bacterial count. Blood sample also be collected on day 0, 7, 14 and 28 of experiment from wing vein with the help of tuberculin syringe in sterile vial under aseptic condition to evaluate the haemato-biochemical parameters i.e. Hb%, SGPT, SGOT, ALP, Urea and Creatinine. After 28 days of experiment, at the age of 50th day, the birds were slaughtered to collect tissue sample from heart, lung, liver and kidney. Illeo-caecal junction tissue was also collected to examine the illeo-caecal coliform count. Fecal coliform count decreased significantly in RM treated group in comparison to control group. There was no significant (P< 0.01) alteration in hemoglobin percentage, uric acid and creatinine level but serum SGPT, SGOT and ALP increased significantly in RM treated group. The illeo-caecal coliform count was decreased significantly in standard control and Rasa Manikya treated groups in both dilutions 1:50 and 1:100 in comparison to normal control group. The result indicate that the test drug Rasamanikya able to decrease the fecal and illeo-caecal coliform count but histopathological findings clearly showed the drug causes chronic toxicity in birds of RM treated group.

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