Flower visiting insects attract by the flowers colour, shape, size and fragrance as pollinator. This is a mutual relationship between flowers and insects. The present study aims to know behavioural response or ethology of flower visiting insect in relation to particular flower and/or probable atmospheric changes in two managed parks, Kolkata, India. The study areas were selected as per heavily-populated neighborhoods, nearby office buildings, nearby roads and continuous vehicular movements, human interactions as visitors. The study was carried out at 2 sampling stations viz (i) Elliot park and (ii) Agri-Horticulture Society. The flower species were selected viz. Helianthus annuus (sunflower), Petunia sp. (petunia) and Buganvilia spectabilis (Buganvilia) planting above mentioned areas because these species are more common among other species. In each flower, behavioural response or ethology of visiting insects were studied by visual observation and total 10 flowers of each species were observed randomly. The present results clearly indicate that various insects were majorly showed foraging and feeding behaviour and only mating behaviour was found in two species. This study is a preliminary assessment of flower visiting insects' ethology but further researches are needed in relation to pollination efficiencies of flower visiting insects in the particular flower and air pollution load nearby area by using instruments. It was concluded that insect visitors are showing foraging and feeding behavior but only two species were showed mating behaviour, which may be due to the vehicular air pollution because two parks are located nearby roads and continuous vehicular movements were observed.

Behavioural activities in relation to toxicological aspects involve behavioural changes of aquatic organisms under the exposure of a contaminant. The present study aims to know behavioural activities of midge larvae Chironomus sp. at the in-situ acute exposure of different chemicals containing wastewater viz. lead-acid battery industrial effluent, mixed industrial effluent and fresh tap water (chlorinated) as drinking water in comparison to control (dechlorinated) water sample (aged tap water). The Chironomus larvae were kept in three different experimental chambers (perforated wall test vessels) with the exposure of different water samples. These samples were made with no dilution, 50 % dilution, 2.5 % dilution and control water sample. The behavioral activities for larvae of Chironomus sp. were measured at 0h, 2h, 24h and 48 h in in-situ condition. The behavioral activities viz. crawling, looping, ventilation, paralyses and subsequently death of the larvae were recorded in the field condition. A significant differences (P < 0.05, P < 0.01 and P< 0.001) were observed with increasing time of exposure while in few cases the data were increased without significance level. It was recorded that after exhibiting behavioural activities viz. crawling, looping, ventilation and paralyses finally all species were died 100 % of the population in lead acid battery effluent following both 24h and 48hr exposure. In addition, death of larvae were 70 % in mixed industrial effluent and 50 % in fresh tap water (chlorinated) after 48h exposure as compared to control sample water. In conclusion, the present results indicate that the larvae of Chironomous sp. are suitable indicators in the evaluation of the effluent quality in the studied stream, potential to know by behavioural toxicological study for heavy metals and organic pollution. Although it is a preliminary observation by assessing behavioural toxicology but future study in relation to biochemical and genetic damage of Chironomous larvae with the exposure of toxic water samples will provide bigger view.

Behavioural activities in relation to toxicological aspects involve behavioural changes of aquatic organisms under the exposure of a contaminant. The present study aims to know behavioural activities of midge larvae Chironomus sp. at the in-situ acute exposure of different chemicals containing wastewater viz. lead-acid battery industrial effluent, mixed industrial effluent and fresh tap water (chlorinated) as drinking water in comparison to control (dechlorinated) water sample (aged tap water). The Chironomus larvae were kept in three different experimental chambers (perforated wall test vessels) with the exposure of different water samples. These samples were made with no dilution, 50 % dilution, 2.5 % dilution and control water sample. The behavioral activities for larvae of Chironomus sp. were measured at 0h, 2h, 24h and 48 h in in-situ condition. The behavioral activities viz. crawling, looping, ventilation, paralyses and subsequently death of the larvae were recorded in the field condition. A significant differences (P < 0.05, P < 0.01 and P< 0.001) were observed with increasing time of exposure while in few cases the data were increased without significance level. It was recorded that after exhibiting behavioural activities viz. crawling, looping, ventilation and paralyses finally all species were died 100 % of the population in lead acid battery effluent following both 24h and 48hr exposure. In addition, death of larvae were 70 % in mixed industrial effluent and 50 % in fresh tap water (chlorinated) after 48h exposure as compared to control sample water. In conclusion, the present results indicate that the larvae of Chironomous sp. are suitable indicators in the evaluation of the effluent quality in the studied stream, potential to know by behavioural toxicological study for heavy metals and organic pollution. Although it is a preliminary observation by assessing behavioural toxicology but future study in relation to biochemical and genetic damage of Chironomous larvae with the exposure of toxic water samples will provide bigger view.

The trees support many bird and animal species while the survival of urban-dwelling species e.g. bird species dominate over others. This particular Nature park in Kolkata is the habitat of birds and fish species suggesting a better environment for food chain maintenance. The Asian open bill stork, Anastomus oscitans among other bird species is a common and provides ecosystem health indicator. The present study aims to understand the occurrence and population nos. of Asian open bill stork on particular tree species surrounding wetlands at Nature Park, Kolkata, India. Our results clearly indicate that a total population of this bird species having 671 nos. on the particular trees but not in all other available trees in the study area. In conclusion, present research emphasizes a novel occurrence and higher population of the A. oscitans being observed on the basis of the presence of particular tree species, though no attempt has been made on physico-chemical properties of present water and microclimate within the park. Therefore, further research planning should be oriented considering the physico-chemical impacts as well as local microenvironment along with human interaction as visitors in such a way so that these barriers cannot affect nesting and breeding of those species as eco-indicator. Also further relevant research is needed to emphasize on the conservation of these specific tree species to increase population of Asian open bill stork, A. oscitans in Nature park.