Isolation of the promoter of Psp68 Pea genomic DNA library was prepared by digesting genomic DNA with different restriction enzymes (EcoRV, DraI, PvuII and SspI) in 4 separate tubes to generate blunt ends of genomic DNA. The digested genomic DNA was purified and further ligated into BD genome walker kit. The primary PCR was done by using AP1 as forward (5′-GTAATACGAC
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