Strawberry (Fragaria ananassa Duch), belong to Rosaceae family, is widely appreciated, mainly for its fruit characteristic aroma, bright red color, juicy texture, It is traditionally asexually propagated by rooted runners. To improve the strawberry varieties this method was not suitable due to incidence of many serious diseases infection resulting in the gradual degeneration of cultivars performance, so micropropagated strawberry plant has been introduced to prevent most of the plant and soil transmissible diseases. Micropropagated strawberry has several advantages, such as its ability to multiply virus free stock rapidly and, the improved capacity of these plants to produce runners for planting in the field. In this study, it was aimed to examine the interaction of BA, IBA and AgNO 3 combinations at different micropropagation stages. Results showed the addition of AgNO 3 to MS medium led to increase shoot number, shoot length, fresh and dry weight at multiplication shoots stage, shoots rooting % and root number at rooting stage. Survival plants %, plant height, leaves number and dry weight at acclimatization stage.