The present work includes synthesis and characterization of some novel 1,3,4-oxadiazole derivatives and the evaluation of the antibacterial activity of the synthesized compound against pathogenic isolated gram-negative and gram-positive bacteria. The activity result showed that some compound exhibited efficient effect against these bacteria. Some compounds had significant inhibition zone against Escherichia coli, pseudomonas aeruginosa and Staphylococcus aureus. The compound OXD3 and OXD4 gave inhibition zone against resistant pseudomonas aeruginosa while stander drug cefepime doesn’t give an activity.