Mite (Tetranychus urticae) that attacks the cassava plants during dry season can reduce the yield up to 53%, depending on plant age and duration of attacks. The objective of the trial was to evaluate the cassava promising clones for tuber root yield and mite resistance. The field trial was done in Malang, East Java, Indonesia, in 2018 with fifteen clones using a randomized complete block design, with three replications. The glass house experiment for mite evaluation was done in Malang in 2018. A total of fifteen clones were used in this glass house trial. Infestation of mite (imago) was done 1 month after plating with 15 mites/pot on the lower part of the fourth or fifth leaf. Results of the field experiment showed that there was a genetic variability in the clones tested. The fresh tuber yield in 10 months ranged 30.33–55.67 t/ha with mean 41.34 t/ha. The fresh tuber yield of clone OMM 0915-11 was the highest of 55.67 t/ha. The tuber result of clone UJ5d50-207-3 similar to OMM 0915-11 and significantly higher than check variety UJ5. Response of clones to mite attack were as follows: two clones were resistant, ten clones were moderately resistant, and the other clones were susceptible. Based on the green house trial, the response of clones to mite attack was as follows: one clone was highly resistant, two clones were resistant, ten clones were moderately resistant, and the other clones were susceptible. Clone OMM 0915-11 was resistant variety or high resistant variety based on the green house and field experiments, while clone UJ5d50-207-3 was moderately resistant based on both glass house and field experiments.

Background: Knowledge about genetic parameters can help plant breeders to determine which selection criteria are beneficial. This study aims to estimate the genetic parameters of soybean seed characteristics.Methods: A total of 91 soybean lines from three different populations were evaluated during dry season in 2016. All genetic material used was grown following a randomized complete block design with two replicates. Soybean pods were harvested after 90% of the leaves had yellowed or fallen. The characteristics of the seeds observed included length, width, thickness and weight of 100 seeds.Result: The results showed that there were two seed shapes (round and ellipse) and two seed sizes (large and medium). There is a broad genetic variability of soybean seed characteristics. Heritability broad sense varies from 0.42 to 0.84, classified as moderate (length-width ratio) to high (length, width, thickness, length-thickness ratio, width-thickness ration and weight of 100 seeds). This therefore shows that there is an opportunity to improve the characters of soybean seeds. The length, width, thickness, ratio of the three characters and the weight of 100 seeds can be used as selection criteria in a soybean breeding program to obtain large-seeded soybeans with a round or elliptical shape.

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Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi jamur endofit pada tumbuhan paku Asplenium nidus. Metode yang digunakan dalam penelitian ini adalah isolasi jamur endofit, pemurnian jamur endofit, dan identifikasi jamur endofit secara makroskopik dan mikroskopik. Hasil isolasi dan pemurnian jamur endofit diperoleh lima isolat jamur endofit yaitu AN1, AN2, AN3, AN4 dan AN5. Teridentifikasi sebagai genus Gliocladium, Neoscytalidium, Humicola, Phialophoroides dan Aspergillus.This study aims to isolate and identify endophytic fungi in Asplenium nidus. The method used in this research is endophytic fungal isolation, purification of endophytic fungi, and identification of macroscopic and microscopic endophytic fungi. Results of isolation and purification of endophytic fungi obtained five endofit fungal isolates are AN1, AN2, AN3, AN4 and AN5. Identified as genus Gliocladium, Neoscytalidium, Humicola, Phialophoroides and Aspergillus.

Kuhnle et al. has reported that Sunkist peels contain 36 µg/100 g wet weight of phytoesterogen. This study was designed to explore Sunkist peels' potential to recover the infertility due to ovulation failure. This study was performed in June 2020 at the Pharmacology Laboratory, Universitas Prima Indonesia, on 25 rats. These rats were divided into five groups: Control, Standard, and Sunkist Peels Extract-I, II, and III that received 1 ml of 0.5% Sodium Carboxymethyl cellulose suspension, ethinyl estradiol 50 g/kgBW, 300 mg/kgBW, 450 mg/k BW, and 600 mg/kgBW of ethanol extract from Sunkist peel, respectively. Every 8-12 hours, the rat’s vagina were swabbed and smear into on object glass to be stained by 10% Giemsa and viewed at 40x and 100x magnifications. The leucocytes, especially neutrophils, epithelial cells, cornified and epithelial cells as well as the density of cells in vaginal swab were evaluated to determine the estrus cycle of the rats. The length of estrus phases was express as median (IQR) and analyzed using the Kruskall-Wallis test. The result of this study showed that the standard and highest dosage extract showed a significant extending of estrus and metestrus phases compared to the control group and significantly shortened diestrus phases. Hence, the highest dosage of Sunkist extract can prevent infertility by increasing the period of estrus cycle (ovulation) at the same level as the standard group.