The effect of ethyl acetate fraction of paku resam leaves (Gleichenia linearis (Burm. Clarke) on endothelial cell dysfunction of white male mice has been done. Dysfunction of endothelial cells was induced by sodium chloride (NaCl) at a dose of 28 mg/kg BW. Ethyl acetate fraction G linearis administered orally at the dose of 35, 70 and 140 mg/kg BW together with NaCl for 14 days. The endothelial cell dysfunction was observed by nitrous oxide (NO) serum levels. The results showed an increasing level of NO in mice serum given the paku resam extract with a dose of 35, 70 and 140 mg / kg BW with increasing percentage of NO levels at 85.66; 62.23 and 48%. After being analysed by one-way ANOVA only the dose of 35 mg / kg BW of the positive control showed the levels of NO significantly different (p <0.05). It can be concluded that ethyl acetate fraction of G linearis at a dose of 35 mg / kg BW has the best effect to prevents the endothelial cell dysfunction in mice.