Background: Thyroid dysfunction in pregnancy is clinically important as insufficient thyroxine is associated with an increased risk of premature birth, low birth weight and miscarriage. This prospective, cross sectional study, single-center study was carried out to identify the thyroid dysfunction in pregnancy in Indian antenatal mothers.                                                                                                            Methods: This prospective, cross sectional study, single-center study was conducted in the Department of Obstetrics and Gynecology, Indian Institute of Medical Science & Research, Warudi, Badnapur, Jalna, India. The study period was of 20 months from January-2018 to August-2019. One hundred and sixty six antenatal mothers with age population in range of 18-35 years were included in the study.  All the study subjects were randomly selected & rendered to serological testing’s like T3, T4, TSH. All The study protocol was performed in accordance with the principle of the declaration of Helsinki and after approval by the Institutional ethical scientific committee. The data was entered and tabulated in MS-Excel 2007, and statistical analysis was performed by using Statistical Package for the Social Sciences (SPSS 22.0). Data had been summarized as mean for numerical variables and count and percentages for categorical variables.