Chemical analyses, polyacrylamide-gel electrophoresis and isoelectric focusing of milk-fat globule membrane proteins (FGMP) obtained from the milk of 2 Murrah buffaloes were done to determine if any change in composition occurred during lactation. Changes in the levels of sialic acid, hexose, hexosamine, N and P were found in the FGMP obtained at different stages of lactation. On the day of parturition, 8 major proteins in FGMP were determined by sodium dodecyl sulphate polyacrylamide-gel electrophoresis whereas 6 major proteins were obtained in FGMP of middle and late lactation milks. Isoelectric focusing of FGMP showed 8-9, 9-13 and 13-16 proteins from colostrum, middle and late lactation milks, respectively and the isoelectric pH of the proteins varied from 5-25 to 7-80, 5-85 to 8-30 and 5-75 to 8*61 respectively.
The present paper considers t h e comparison o f t h e double sampling d i f f e r e n c e e s t i m a t o r given i n Singh and Singh (1979) f o r random non-response and t h e simple random sample mean w i t h respect t o these concepts. The empirical study shows t h a t t h e r e l a t i v e e f f i c i e n c y and a l s o t h e r e l a t i v e non-response robustness o f t h e simple sample mean ( w i t h respect t o the d i f f e r e n c e estimator) are decreasing f u n c t i o n s o f t h e r a t e o f random nonresponse.
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