Securing water resources is a complicated issue in Kazakhstan. Only 36% of Kazakhstan’s rural population has access to a centralized water supply and 57.3% use groundwater accessed by wells and boreholes. The groundwater quality must be monitored to minimize health risks. The aim of this project is to investigate the groundwater quality in the Zhambyl region of Kazakhstan. Groundwater depletion, pollution, waterlogging, and salinization are all widespread in Kazakhstan. Previously, 500 self-flowing and, within this project, 204 wells were investigated in southern Kazakhstan, the Zhambyl region. The field works and data processing was carried out in three phases: first, a fieldwork survey of existing water wells; the second phase, field work with more detailed hydrogeological investigations, including measurements of flow rates, pH, temperature, and electrical conductivity of water samples; the third phase, processing, and analysis of field data samples in chemical laboratories. Kazakhstan’s requirements for drinking water are much lower than the requirements in the EU. Less than 30% of Kazakhstan’s population has access to safe water and about 50% of the population consumes drinking water that does not meet international standards of salinity, hardness, or bacteriological levels.