Caralluma lasiantha is a succulent plant belongs to Ascalpedaceae family. The objective of this study was to evaluate and to compare the anti-hyperglycemic effect of Caralluma lasiantha with Chromium Picolinate on hyperglycemia induced by Cafeteria-Diet in Wistar albino rats. Hyperglycemia was induced in experimental rats by feeding Cafeteria-Diet for a study period of 90 days. Caralluma lasiantha (10, 20, and 40 mg/kg b.w.) & Chromium Picolinate (10 mg/kg b.w.) were administered orally once every day throughout the study and serum glucose levels were determined in different experimental days. Treatment with Caralluma lasiantha significantly reduced the serum glucose level in dose dependant manner. Data reveal an appreciated beneficial effect of Caralluma lasiantha on hyperglycemic rats. Caralluma lasiantha possesses anti-hyperglycemic effect, which promisingly support the use of Caralluma lasiantha as a food supplement or an adjunct treatment for hyperglycemia INTRODUCTION: Diabetes is increasing at an alarming rate worldwide, which can mainly be attributed to the sedentary life style and calorie-rich diet. Diabetes is often linked with abnormal lipid metabolism and is considered as a major factor for the development of atherosclerosis and cardiovascular complication. 1 Diabetes is a metabolic disorder of multiple etiologies characterized by chronic hyperglycemia with disturbance of carbohydrate, fat, and protein metabolism resulting from defects in insulin secretion, insulin action, or both. 2 Hyperglycemia is a common end point for all types of DM and it is an important parameter which can be assessed to evaluate the effectiveness of anti-diabetic drugs.