Dried Blood Spot (DBS) become a most proficient method of sample collection for both developed and developing countries. It is most commonly using to collect, store and transport sample for serological/immunological test, and pharmacokinetic studies, metabolic profiling etc. DBS has become an alternative tool for long-term storage, less hazardous and cost-effective method of sample handling. Thus, present study designed to investigate the stability of anti-HIV 1 and 2 antibodies in DBS in our environmental setting.