Objective:To determine antimutagenic activity of Cassia auriculata Linn. on chromosomal damage induced by cyclophosphamide (CP).Material and Methods:In the present investigation, four groups of six Swiss albino mice in each group were used. Excepting for thefirst group all the remaining groups were treated with CP (50 mg/kg). Mice of third and fourth group were treated with ethyl acetate extract of C. auriculata Linn. at 100 mg/kg and 200 mg/kg with CP. Metaphase of bone marrow cells of all animals were analyzed for qualitative and quantitative chromosomal aberrations. Break, fragment, deletion, Polyploidy, pulverized, ring and total aberration were observed.Results:Flavonoids rich extracts of root of C. auriculata Linn. provided significant protection (P < 0.05) against CP induced chromosomal aberration. Total chromosomal aberration was found to be 12.16 and 7.33% in 100 and 200 mg/kg of extract treated animals respectively.Conclusion:From the present study it can was observed that ethyl acetate extract of C. auriculata Linn possess significant anti-mutagenic potential against CP induced chromosomal aberration.

The world is observing an unprecedented development in the usage of herbal product at national as well as international levels. This requires the improvement of current and aimed standards for estimating the quality, safety and efficacy of these drugs. The leaves of Ailanthus excelsa and the fruits of Randia Dumetorum are medicinal plants that are used for many diseases around the world. We then collected the flavonoids and saponin fraction extracted from the leaves of Ailanthus excelsa and the fruits of Randia dumetorum. To determine the reliability, quality and purity of these particles, we provide a crucial pharmacological profile along with the antioxidant activity. Pharmacological studies, such as morphological, physicochemical, TLC, and phytochemical analysis of all fractions containing total phenol and flavonoids, were performed according to specific methods. DPPH tests estimated the antioxidant action of all fractions, Hydrogen peroxide scavenging assay, and reducing power assay method. Previous phytochemical studies discovered the occurrence of saponins, flavonoids, tannins, and especially phenolic chemicals. All fractions have antioxidant effects, depending on the existence of a phenolic compound. The above parameters are vital to establishing pharmacological rules for the authentication of Ailanthus excelsa leaves and Randia Dumetorum fruits.

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