To evaluate the effectiveness of ozonation treatment (1/2 ppm for up to 30 min at 3-7 centigrade) on the persistence of multidrug-resistance Staphylococcus aureus (MDR S. aureus) tainting cattle meat. Design: Descriptive study Procedures: Nine testers of cattle's meat including imported (n = 3) and locally slaughtered (n = 6), which were stored at -18 centigrade and accepted positive for MDR S. aureus were subjected to aqueous ozone (O3) as 1/2 ppm for 15 and 30 min. Results:The results presented that after ozonation treatment (1/2 ppm at 3-7 centigrade), the whole testers which free from MDR S. aureus were 22.2% & 55.6% for 15 & 30 min, respectively. Additionally, the antimicrobial effectiveness of aqueous O3 as 1/2 ppm on the decline of MDR S. aureus level (log 10 CFU/ml) was measured against three contaminated testers of cattle's meat. The outcomes publicized that afterward handlings, the total decline of bacterial counts was 2-3 log 10 (CFU/ml) after 30 min at 3-7 centigrade, this decline is extremely noteworthy from the opinion of public health. The effectiveness of aqueous O3 (0.5 ppm/3-7 centigrade) with carcass drip to MDR S. aureus was assessed and the results displayed that 77.8% and 100% of the testers of aqueous O3 were negative after ozonation treatments for 15 and 30 min, respectively. Conclusion and clinical relevance:O3 as 1/2 ppm is exceedingly active in dropping the amount of MDR S. aureus contaminated testers and this decline augmented as prolonged experience time to ozonation treatment. On the other hand, O3 was highly effective in eradicating MDR S. aureus even in the presence of high levels of organic materials. These outcomes designated O3 as a substitute promising approach to decline meat contagion with foodborne microorganisms for instance MDR S. aureus.