NucPNP and NucV involved in the early steps of nucleocidin biosynthesis were characterized in vitro. The results provide insight to study the biosynthesis of nucleocidin in the future.

A new chalcone-based fluorescent turn-on probe (3c) responsive to the nitroreductase (NTR) activity and its application toward the detection of bacteria are presented. The probe exhibits turn-on fluorescence at 535...

A fatty aldehyde surrogate containing a formyl thioester group can be reduced by fatty aldehyde reductase (FALR) with stoichiometric formaldehyde generation. It can be rapidly visualized and quantified using the Purpald assay for screening applications.