Metal nanoparticles synthesis by green route represents most important part of biotechnology that is reliable, simple, cost-effective and eco-friendly as compared to physical and chemical methods. In present study Silver nanoparticles was synthesized using aqueous leaf extract of Lowsonia inermis (Henna). This nanoparticles was used as stabilizing and reducing agent in bioreactors and was observed to be effective than chemicals and physical synthesis for its application in different type of dye degradation. Characterization of synthesized silver nanoparticles was by UV-Visible, Fourier transform infra-red-spectroscopy (FTIR), Scanning electron microscope (SEM), and X-ray diffraction (XRD) techniques. This paper emphases on naturally obtained nanoparticles for the further commercial use for dye degradation and so on.