Cefixime is used for bacterial infections recovery, Ornidazole is used as antibiotic for protozoan infections and Moxifloxacin is also an antibiotic for multiple bacterial infections. Accurate, simple and precise HPLC method was developed for the determination of Cefixime, Ornidazole and Moxifloxacin in the tablet pharmaceutical dosage form. The RP-HPLC method was developed and validated with precision, specificity, accuracy, ruggedness, robustness and linearity. Chromatographic conditions are mobile phase A: 6.8g KH2PO4 in 1000 ml water and mobile phase B: Acetonitrile, Agilent Zorbax SB-C18, 100 x 4.6mm, 5µm, 280 nm, 1.0ml/min, 25 min (gradient program: mobile phase B at 0min 5%, 5min 5%, 10 min 15%, 14 min 15%, 17 min 35%, 20 min 5% and 25 min 5%. All validation results showed the accuracy results and % RSD for test area, %assay values were also within the limits. This HPLC method can be used to analyze the regular product quality control purpose.

A single HPLC method for the determination of four active ingredients viz. aspirin, rosuvastatin, ezetimibe and clopidogrel in less run time is developed. HPLC method was developed and validated. X-terra C18 100 × 4.6 mm, 3.5 μ HPLC column, KH2PO4 buffer (mobile phase A) and acetonitrile (mobile phase B) were used. 20 μ injection volume, 1.0 mL/min flow rate, 230 nm and ambient column oven temperature were applied for separation. Gradient program: at 0 min mobile phase-B 13 %, at 4 min 13 %, at 8 min 44 %, 14 min 57 %, 17 min 57 %, 20 min 13 % and 25 min 13 %. Method validation was performed as per ICH guidance with precision, linearity, specificity, accuracy, ruggedness and robustness. Validation results were satisfactory and this method can be applied for regular drug product manufacturing