Live feed is one of the most important factors of the success of fish farming, especially in fish hatchery activities. Live feed that has the potential to support cultivation activities is Daphnia magna. One of the problems on Daphnia magna culture is feed availability that can meet the nutrients in Daphnia magna. The aim of this research was to study the effect of providing feed from combination of chicken broth and chicken manure on the nutritional value of Daphnia magna. This study was experimental using the RAL method consisting of 4 treatments with 5 replications, namely control treatment (P0) 7.5 mL/L dose of feed, (P1) 2.5 mL/L dose of fermented feed, (P2) 5 mL/L dose of fermented feed and (P3) 7.5 mL/L dose of fermented feed. Data analysis was processed using ANOVA and Duncan. The result of this study has a significant effect (P<0.05) on nutritional content of Daphnia magna with the best dose in P3 which can affect the highest protein (51,98%) and highest fat (12,17%) of Daphnia magna. The highest fiber (13,32%) of Daphnia magna was found in the P1 treatment and the highest carbohydrates (37,51%) of Daphnia magna was found in the P0 treatment.