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(8) with the zinc concentration reduced by a factor of 10, 3% w/v sucrose, 0.1 mg/l of NAA, 100 mg/I of myoinositol, 0.2 mg/l of glycine, 0.5 mg/l of nicotinamide, 0.5 mg/l of pyridoxine, 0.5 mg/l of thiamine, and 0.1 mg/l of p-aminobenzoic acid. Cultures were grown in 250-ml Erlenmeyer flasks with 50 ml of medium, or in 125-ml Erlenmeyer flasks with 20 ml, on a reciprocating shaker (108 strokes/min, stroke amplitude 2 cm) in dim (5 ft-c) light at 25 to 28 C. During the 3-week subculture period, branches formed on inoculated segments, and the branches extended 2 to 4 cm in length. These branches did not form secondary branches within the 3-week subculture period. Segments (1-cm long) cut from the branches in a 3-week-old culture were used as inocula, either for subculturing or, after rinsing with distilled water, in experiments. Three to five segments were used per flask.Assessment of Results. In most of the experiments reported here, the number of branches initiated in a given time interval was the response measured. Segments were generally harvested 1 Abbreviations: NAA: ca-naphthalene acetic acid; BA: benzyladenine.www.plantphysiol.org on May 11, 2018 -Published by Downloaded from