Background: In 1988 the US Food and Drug Administration permitted low dose methotrexate for the treatment of rheumatoid arthritis that would change the progression of the disease. Methotrexate is a folic acid antagonist and its systemic use causes numerous side effects; including hepatic toxicity. It would be preferable to deliver methotrexate by the topical route to reduce side-effects along with ease of administration and reduced dosing frequency. So, nanoparticle gel is a hopeful approach to treat rheumatoid arthritis. Objective: To develop a nanoparticles gel containing novel natural polymer-based methotrexate nanoparticles and evaluate its therapeutic potential on Complete Freund’s Adjuvant–Induced Arthritis rat model and compare it to methotrexate and dexamethasone gel. Result and Discussion: Methotrexate nanoparticles gel significantly reduced the percentage inhibition of oedema compared to methotrexate and Dexamethasone gel. The therapeutic activity of nanoparticles gel was found to be F3W2 ≥ F2W2 ≥ F1W2 ≥ F4W2 ≥ MTX gel. So, the optimized nanoparticle gel formulation F3W2 can be effective in treatment of rheumatoid arthritis. Materials and methods: The five batches methotrexate nanoparticles gel were prepared viz. F1W2, F2W2, F3W2, F4W2 and methotrexate gel for the topical application by using different concentrations of Carbopol 934 base and characterized for their evaluation parameters: Homogeneity, Grittiness, pH, Spread-ability, Viscosity determination, and Drug content studies. The arthritic potential of methotrexate-nanoparticles gel was evaluated by CoResult and Discussion: Methotrexate nanoparticles gel significantly reduced the percentage inhibition of oedema compared to methotrexate and Dexamethasone gel. The therapeutic activity of nanoparticles gel was found to be F3W2 ≥ F2W2 ≥ F1W2 ≥ F4W2 ≥ MTX gel. So, the optimized nanoparticle gel formulation F3W2 can be effective in treatment of rheumatoid arthritis.mplete Freund’s Adjuvant–Induced Arthritis rats model based on percent inhibition oedema and arthritic score. Conclusion: The developed novel nanoparticles gel formulation can be a promising alternative to existing methotrexate and Dexamethasone gel.

Deccan hemp is rich in mucilage and of immense value. This study was performed to examine the mucilage of Deccan hemp and its (Gas Chromatography-Mass Spectroscopy) analysis was performed to identify the presence of sugar composition in mucilage for the development of pharmaceutical formulation. Mucilage was found to be 9.54% w/w which was off white in colour, tasteless and with characteristic odour. Physicochemical characterization revealed that mucilage has enough moisture that is 9.34 % w/w and is of neutral pH. It was found to be soluble in hot water and insoluble in organic solvents while in cold water mucilage swelled to form a gel. The GC-MS analysis of mucilage showed the presence of glucose, fructose, sucrose, maltose and xylose that scope to be of scientific relevance particularly plant polymer based excipient and coating material in pharmaceutical products. The present investigation showed that Deccan hemp mucilage has high pharmaceutical significance it can be used as excipient and coating material in pharmaceutical formulation.

Deccan hemp is rich in mucilage and of immense value. This study was performed to examine mucilage of the plant and its functional group with the help of FT-IR Spectroscopy for preparation and development of pharmaceutical formulation. Mucilage was found to be 9.54% w/w which was off white in colour, tasteless and with a characteristic odour. Physicochemical characterization revealed that mucilage has enough moisture i.e. 9.34 % w/w and is of neutral pH. It was found to be soluble in hot water and insoluble in organic solvents while in cold water mucilage swelled to form a gel. FT-IR analysis of mucilage showed the presence of -as major markers that scope to be of scientific relevance particularly plant polymer based excipient and coating material in pharmaceutical products.