Today, seaweed has become essential to medicine due to its ability to treat disease-afflicted cells without majorly influencing healthy cells and tissues. This study included a phytochemical analysis, in vitro antioxidant and antidiabetic activity of all the extracts of Botryocladia leptopoda. Also, analyse the cytotoxicity and glucose absorption activity of the ethanolic extract of B. leptopoda using 3T3 cell line through MTT assay. The extraction of secondary metabolites from B. leptopoda using various solvents (hexane, chloroform, ethyl acetate, ethanol and water) resulted in alkaloids, Phenol and Flavonoids. The ethanolic extract of B. leptopoda showed the best in vitro antioxidant and antidiabetic activity compared with all the experimented extracts. Ethanolic extract exhibited no cytotoxicity and improved glucose absorption. This study has revealed that marine red alga B. leptopoda showed noticeable in vitro antioxidant, antidiabetic activities and no toxic effect toward normal cells. We suggested to carry out the antidiabetic activity through in vivo investigations on Botryocladia leptopoda (J.Agardh) Kylin.