Herbal medicine (also Herbalism) is the study of pharmacognosy and the use of medicinal plants. Plants have been the basis for medical treatments through most of human history, and such traditional medicine is still widely practiced today. Archaeological evidence indicates that the use of medicinal plants dates back to the Paleolithic age, approximately 60,000 years ago. Written evidence of herbal remedies dates back over 5,000 years to the Sumerians, who compiled lists of plants.

The analytical method was developed and validated for determination of Alogliptin in bulk and pharmaceutical dosage forms by High performance liquid chromatography. The separation was carried out on Zorbax SB-Aq (250 x4.6mm,5µ ID) column. The mobile phase consists of 0.1% TFA Water : ACN in the ratio 62:38 at flow rate 1ml/min with diode array detector wavelength at 290nm.The column temperature was adjusted at 30º ± 0.5°C with injection volume 10µl.The retention time of Alogliptin was 3.06min. The linearity of the calibration curve was linear over the concentration range 25-75µg/ml (r2=1). The validation was carried out as per ICH guidelines. The development of method was easy, rapid, linear, precise, accurate and consistent.

Objective: A new, simple, sensitive, precise and reproducible UV spectroscopic method was developed for the estimation of Lignocaine Hydrochloride in bulk and Semisolid Formulation. Methods: The UV spectrum of Lignocaine Hydrochloride in RO water showed λ max at 228.8nm. Beer’s law is valid in the concentration range of 20-100µg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 20-100µg/ml with regression equation y = 0.0097x + 0.023 and regression correlation coefficient r2= 0.9992. Moreover, the method was found to be highly sensitive with LOD (3.55μg/ml) and LOQ (10.75μg/ml). Conclusion: Depending on results the given method can be successfully applied for assay of Lignocaine Hydrochloride in Semisolid formulation.