Summary. RNA w;is prepared from culuired I'lasmodium falciparum usinĝ ibonllcleo^ide-va^adyl rihoniickase inhibitors and assayed in a rabbil lelicnlocyie, cell-free lysate iranslation system. Discrete proteins, ranging from < 10.000 lo >20(),000 daltons, were synthesized. Some of these proieins were precipitjited by immune serum and were similar to those metabolically synthesized. Purified poly [A] mRNA was also translated and difTerences between the fractions which either bind or pass ihroiigh ;i poly [U] Sepharose column ;ire described.

Summary.Proteins from synchronized continuous cullures of Ptasmodium falciparum were biosynthetically labelled with [•'•'''S]methionine at ring, trophozoite, and schizont stages. Several proteins appeared to be synthesized predominantly at one stage only, especially the schizont stage. Immunoprecipitation using inhibitory human sera revealed that a variety of proteins, from all stages of parasite growth, were recognized. More proteins, especially those with high molecular weight, were precipitated from the schizont stage than from ring and trophozoite stages, but two major proteins (MW -70,000 daltons and --45.000 daltons) were recognized in all three stages.

Streptococcal infection is a main infectious diseases for farmed grey mullet (Mugil cephalus). This study were to identify spreptococcal species in diseased farmed grey mullet and to investigate differences in susceptibility to 13 antibiotics and in genotypes between the stains from the grey mullet and non-grey mullet. 170 samples from diseased farmed grey mullet were collected from three county in 2013 -2016. Multiplex PCR identified L. garviea (146) as the main pathogen, S. agalactia (9), S. dysgalactiae (19), and double infection (5), but no S. iniae. The prevalence changed annually and differed among three counties. Pulsed-field gel electrophoresis (PFGE) analysis demonstrated identical genotype with an ApaI-digested DNA pattern. Disc diffusion results demonstrated differences in antibiotic susceptibility between the strains from grey mullet (146) and non-grey mullet (30). Almost all strains resisted to clindamycin and all strains were susceptible to six antibiotic in grey mullet and 4 antibiotics in non-grey mullet. The reduced susceptible strains was more in non-grey mullet than grey mullet group. The reduced susceptible strains were observed the highest in 2014 and in Chiayi county and decreased from 2014 to 2016. However, the strains with reduced susceptibility to ceftriaxone, cirpofoxacin, moxifloxacin, tetracycline for human treatment were observed.