Background. Isoproterenol is a synthetic catecholamine that can produce diffuse myocardial necrosis at high doses. Advanced glycation end products (AGEs) are involved in the development and progression of cardiovascular disease, including acute coronary syndrome. The purpose of the study was to determine the changes of serum and tissue AGE content in isoproterenol-induced acute myocardial infarction and to assess their informational character in the early diagnosis, risk stratification and prognosis of disease. Methods. Forty adult male rats were divided into 5 groups: sham (L1=11), control 0.9% NaCl (L2=11), and with experimental myocardial infarction (L3=6, L4=6; L5=6), induced by the subcutaneous injection of Isoproterenol Hydrochloride solution 100 mg/kg, and sacrificed over 6 hours, 24 hours and 7 days post infarction. The results were presented by median and interquartile range. The groups were compared using Kruskal-Wallis and Mann-Whitney nonparametric tests, and the Spearman correlation coefficient was calculated (SPSS 23.0). Results. A decrease of AGE serum levels in L3 were identified, followed by a significant increase in L4, the trend maintained in L5, which significantly exceeded the values in sham and control groups. In the homogenate, AGEs presented an elevation in L3, with a relevant decrease in L4, and an inconsistent increase in L5 compared to sham and control groups. Conclusion. The collected data suggest the utility of AGE assessment in early diagnosis and risk stratification in acute myocardial infarction.

The objective of our research was to study the effect of controlled reperfusion on lactate and advanced oxidation protein products (AOPP) in experimental ovarian torsion (OT), and to evaluate the possibility to reduce the reperfusion injuries applying the ON-OFF technique of reperfusion. Material and Methods — Lactate and AOPP were measured in serum and ovarian homogenates of 70 females rats, divided equally into 7 groups: no intervention (nr. 1); laparotomy (nr. 2); 3 hours OT (nr. 3); 3 hours OT + 1 hour (nr. 4) or 24 hours (nr. 6) of simple reperfusion (SR); 3 hours OT + 1 hour (nr. 5) or 24 hours (nr. 7) of controlled reperfusion (ON-OFF). The results were analyzed using Welch's ANOVA. Results — OT produced an increase of serum (66%, p<0.001) and homogenate (38%, p=0.006) lactate, that tended to return to baseline in 24 hours despite the type of reperfusion. AOPP had grown in OT animals serum (20%, p=0.007) and homogenate (26%, p>0.05) and continued to rise in the serum after SR, while in ON-OFF technique groups – decreased by about 20% (p<0.01). Homogenate AOPP levels rised after reperfusion in all groups. Conclusion — Lactate is a biomarker for ischemia in OT. Increased AOPP in experimental groups indicates that there is a degree of oxidative stress that can affect the cells after the restoration of the blood flow. Therefore, the correct management of the condition that reduces reactive oxygen species production, such as controlled method of reperfusion, could be appropriate to prevent additional injuries.

The administration of new Schiff heterocyclic bases and their copper (II) complexes, thiosemicarbazide derivatives induces various influences on the activity of glutathione-dependent enzymes in splenic tissue. The selective action of the studied compounds on the expression of glutathione enzymes is evident, which is manifested by a tendency of insignificant decrease of glutathione reductase (GR) and glutathione peroxidase (GPO), insignificant increase of glutathione-S-transferase (GST) and marked depression of glutaredoxin levels. The slight increase of GST under the action of the studied compounds can be considered as a protective reaction aimed at stagnating the intensity of peroxide processes and supporting the activity of the glutathione cycle, and decreased the activity of glutaredoxin denotes their antiproliferative, cytostatic effect. Further studies are needed to evaluate the mechanisms of action of the investigated compounds on the activity of glutathione-dependent enzymes in other biosystems and animal experiments, to assess the practical importance of the obtained results.

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