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The effect of fluorescence saturation under laser pulsed excitation appears as a non-linear response of emission signal versus intensity of excitation. The saturation becomes important when the rate of molecular excitation exceeds the rate of their deactivation. Typically the effect is noticeable when excitation photon flux (p) exceeds 1023... 1024 cm2s'. We present the theory offluorescence saturation under laser pulsed excitation for both quasi-stationary and non-stationary cases, as well as the experimental study on fluorescence saturation for of organic dyes in aqueous and ethanol solutions and in polymer film.

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el (39J-flZ-23996i10, fa (39)-02-239%126, cmoii: rinoldo e u~~i p a /~m i , i rWe present an innovative appmaeh 10 photon mimalion in a m l a y a System The raonsbuction of