Objectives: Since its discovery, the role of histamine in inflammation is controversial; thus, according to some authority, it is mainly pro-inflammatory, and according to others, it is anti-inflammatory in nature. In this scenario, we thought that the contradictory results are dose dependent, thus in this study, our aim was to find the specific role of ultra-diluted histamine in pulmonary inflammation. Materials and Methods: Ultra-diluted histamine ( ~1 pg/ml) was administered in chick lung hypoxic inflammation in an restricted organoid culture along with lysozyme, ovalbumin, and blank controls. Results: The ultra-diluted histamine showed a significant role as an anti-inflammatory and bronchodilator agent and the anti-inflammatory action was found similar to lysozyme. Conclusion: Ultra-diluted histamine may be used as an anti-inflammatory agent.