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Seaweeds (Marine macroalgae) area large group of marine organisms containing important phytochemical constituents with various biologicalactivities .They are potentially prolific sources of highly bioactivese condary metabolites, which manifest many of the rapeutic effects like anti-cancer, anti-oxidant, anti-inflammatory and anti-diabetic activities. Seaweeds are used by many Asian cultures fortraditional medicine preparations. The Caulerpapeltata was collected from Rameshwaram coastal area it was shadedried, madein to powder using standardized procedure to get Caulerpapeltata Methanolic Extract (CPME). The phytochemicals and Gas Chromatography-Mass Spectrometry (GC-MS) analysis was done on prepared CPME for identifying the bioactive compounds Phytochemical in vestigation suggests that the Caulerpapeltata exhibited the presence of phytochemicals like Alkaloids, Carbohydrates, Phytosterols, Saponins and Diterpenes,which may contribute to its biological activities. GC-MS analysis showed 28 variety of compounds,among which Dibutylphthalate, n-hexadecanoic acid, and1,2–Benzenedic arboxylic acid was found in high percentage. The phytochemical studies and the compounds available in GC–MS showed that the Caulerpapeltata contain important bio active compounds,which may have anti-microbial,anti-fungal and anti-canceractivity. Further research is needed for finding its use in development of new pharmaceutical agent and its safe consumption by human for various health benefits.

Introduction: Caspase 3, an apoptosis executioner, inhibition may be beneficial for diabetes, nephropathies, neurodegenerative disease treatments and in areas of regenerative medicine. Since early traditional medicine, plant extracts comprised the major treatments of many ailments. Phytoconstituents have been a prime source for therapeutics, which are abundantly available resources. Therefore, with the interest to identify potential anti-apoptotic agents in plant extracts, D-galacturonic acid (DGA) was selected for screening anti-caspase 3 activity as it is the major constituent in Momordica charantia (bitter melon) and many other fruits’ pectin composition. Objectives: The present study aimed to evaluate activity of major phytoconstituent of M. charantia extract, DGA against caspase 3. Materials and Methods: The chemical structure of the ligand was from obtained PubChem database, and the protein structure was procured from PDB database. Molecular docking study was performed using AutoDock version 4.2. Results: This study states the interactions of DGA with GLU’124, LYS’137 and ARG’164 amino acids of caspase 3, where GLU’124, LYS’137 amino acid interactions are important for stability of caspase 3 enzyme. Conclusion: The interactions between DGA and caspase 3 revealed in this study may be helpful in characterizing the medicinal property of this phytoconstituent in the bitter melon extract by future studies.

Purpose: The study aimed to assess the acute toxicity and anti-diabetic activity of Halimedagracilis (green marine alga). Methods: The Halimedagraciliswere collected from the coastal area of the Gulf of Mannar biosphere reserve and shade dried. Methanolic extract of Halimedagracilis (MEHG) was prepared and it was screened for acute toxicity and anti-diabetic activity in the Zebrafish model. In the Acute toxicity study, the Zebrafishes were grouped into 6 groups and dosed with 6.25, 12.5, 25, 50, and 100mg/L of MEHG and observed at 0, 24, 48, 72, and 96 hours’ intervals. Foranti-diabetic activity analysis diabetes was induced using streptozotocin (STZ). The Zebrafish were divided into six groups- control group, positive control, diabetic Zebrafish with three doses of MEHG, and standard control (treated with metformin). Results: Acute toxicity studyshowed no significant behavioral changes and LC50 was determined as 100mg/L. In the diabetic study, test groups when compared to the control group showed: a significant reduction in both fasting and postprandial blood glucose levels and significant changes in the regeneration of pancreatic β-cells, and reduced vacuolization in the islets of Langerhans. Images of the regenerating caudal fins taken at 24, 48 and 72-hours post-amputation displayed significant limb regeneration in MEHG treated fish compared to the control group. Conclusion: These results prove that MEHG in STZ- induced diabetic Zebrafish possess potent anti-diabetic action by ameliorating blood glucose regulation, promoting pancreatic cell regeneration,minimizing long-term diabetic complications bypreventing the emergence of metabolic memory but no behavioral changes.