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Urban green space (UGS) provides multiple cultural ecosystem services (CES) and enhances people’s physical and mental well-being. The importance of UGS was more critical during crisis periods such as the COVID-19 pandemic. Recently, UGS has been under severe threats due to rapid urban expansion and climate change. Particularly, the cities in developing countries have experienced a dramatic loss of green spaces due to land use and land cover (LULC) change. This study aims to assess the impact of the COVID-19 pandemic on CES perception obtained from two public UGS, i.e., Subhankar Sishu Uddyan (SSU) and Bandh Road (BR) in English Bazar Urban Agglomeration (EBUA), Eastern India. A survey was performed to assess the valuation of CES, CES obtained from UGS, and the use of CES difference during partial (unlock phases) and after lockdown periods. The non-parametric tests were carried out to understand the significant differences between CES and UGS perceived by the respondents. Explanatory factor analysis was also performed to find the association among CES for each UGS. The results reported substantial variations of UGS use during partial and after lockdown periods. During the after-lockdown period, the UGS mainly were used for social relations (51%), spending time with relatives and friends (25%), spending time with children (11%) in SSU and walking (25%), spending time with relatives and friends (21%), and physical activities (17%) in BR. During the partial lockdown period, a significant difference between SSU and BR was recorded from social relations and health value among all CES. After the full lockdown period, a significant difference was observed in recreation, social relations, health, and aesthetic value. The frequency and duration of UGS use during partial lockdown periods substantially increased. About 12% of the total visitor visited UGS daily after the lockdown, whereas it was 4% during the partial lockdown. The study’s findings will assist urban planners and policymakers in understanding the need for UGS to promote the well-being of the citizens. Supplementary information The online version contains supplementary material available at 10.1007/s11356-023-26919-0.

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