We want to present an extensive work on physico chemical parameters of water samples of Mula river at Pune (Maharashtra). Water samples under investigations were collected from Khadkwasla dam to Sangam Bridge during pre monsoon (April May2016) monsoon (July-August2016) and post monsoon (October-November2016) seasons. The observed values of different physico parameters like pH, DO, BOD, COD, Chloride, Nitrate, Sulphate, Calcium, Magnesium and Hardness, etc. of samples were compared with standard values recommended by world health organization (WHO). The Mula-Mutha River water in Pune had deteriorated in quality. Its biological oxygen demand, an indicator of organic pollution, has risen to over 30 mg/l, more than ten times t permissible limits for bathing. Municipal Corporation is currently supplying water sufficient for the projected population. More water means more sewage, beyond the quantum its sewage treatment plants can treat. As a result there is greater pollution loads discharged in to the Mula-Mutha, two rivers that confluence within city limits and serve to flush away Pune's excreta. All the physico-chemical parameters for pre monsoon, monsoon and post monsoon seasons are within the highest desirable or maximum permissible limit set by WHO except some of the parameters like DO, BOD,COD, chloride, calcium, magnesium and hardness.