ABSTRACT:The present study aims to determine the impact of gamma radiation on the germination % and mitotic indices in three varieties of Dolichos lablab L. viz. Arkajay, Swarna uthkrishit and RP-08-31, at different doses of gamma rays i.e. 100, 200, 300, 400 and 500 Gy. Through the acquisition of the following experiment, an attempt to screen out the superior variety among the three selected varieties was done for Dolichos lablab. Among the three varieties studied, Arkajay showed the highest germination % and active mitotic index (AMI). A significant decline in AMI and increment in total abnormality percentage (TAB%) was observed with the increase in gamma rays from lower to higher doses. TAB% was induced due to the ionizing radiation. Stickiness, precocious movement, laggard and unorientation were found as preponderant chromosomal abnormalies. However, stickiness was the major abnormality at both metaphase and anaphase stages of cell division. In the present study, variety Arkajay emerged as the best variety based on the fact that it showed highest germination percentage and AMI among all of the three varieties selected.