The increase in incidence and affected region of dengue hemorrhagic fever (DHF) in Banda Aceh poses a high risk for health disaster or outbreak. Incidence of DHF in Banda Aceh tends to increase every year. Free-flick number in 11 sub-districts within Banda Aceh city are yet 69.1%, far lower than the national standard. Thus, the preparedness of health workers in sub-district public health center dealing with health disaster due to DHF must be improved. This study aims to analyze the effect of the characteristics of health workers working in the sub-district public health center and endemicity status to preparedness in the face of DHF outbreaks in Banda Aceh City. The research design used was an analytical survey with a sample of all health workers in 11 (eleven) health centers in the city of Banda Aceh, totaling 165 people. Data collection was done using a questionnaire and analyzed by univariate, bivariate and multivariate. The results showed that gender, length of service and training partially related to preparedness of health workers in dealing with DHF outbreaks. It was found that gender, job title, duration of duty and training is partially related to the preparedness of health workers in dealing with DHF outbreaks. The analysis results from binary logistic regression show that age, duration of duty and training are significant predictors of the preparedness of health staff in the Public Health Centre in dealing with DHF outbreaks. The result also shows that the older the health worker age, the more prepared in dealing with DHF outbreak. Similarly, the longer duration of duty the better prepared the health workers on the face of DHF outbreak. It was also found that training is the most dominant predictor affecting the preparedness of health workers in dealing with DHF outbreak. Based on these results, it is suggested to Banda Aceh Health Office to facilitate and advocate training activities related to DHF countermeasure by providing the necessary budget. It is also suggested that the transfer of medical workers should consider and maintain employee with long duration of duty due to their experiences in facing the DHF outbreak.