Controlled the formation of monolayer or multilayers of RCs on a gold electrode Correlated electrochemical and photocurrent responses to the RC adsorbed conformations measured using AFM Adsorbed RC conformations affect photocurrent generation Photocurrent-potential response influenced by local redox mediator concentration and pH environment experienced by the adsorbed RCs

Summary The formation of defined surfaces consisting of photosynthetic reaction centers (RCs) in biohybrid solar cells is challenging. Here, we start with the production of engineered RCs for oriented binding. RCs are deposited onto gold electrodes, and 6-mercapto-1-hexanol (MCH) is used to displace multilayers and non-specifically adsorbed RCs. The resulting electrode surfaces are analyzed for photocurrent generation using an intensity-modulated light and lock-in amplifier. Atomic force microscopy (AFM) is used to characterize the surface and the formation of RC structural assemblies. For complete details on the use and execution of this profile, please refer to Jun et al. (2021) .