No abstract

In Streptomyces lividans, a conditional lethal mechanism was obtained by inactivation of gylB in the glycerol operon. The resulting strain was efficiently counter‐selected on glycerol‐containing medium but the mutation had no effect on the capacity of the strain to be maintained in non‐sterile soil samples and to transfer derivatives of the genetic element pSAM2. However, despite the efficiency of the donor counter‐selection system, the transfer of a pSAM2 derivative from S. lividans to indigenous soil bacteria was not detected.

In Streptomyces lividans, a conditional lethal mechanism was obtained by inactivation of gylB in the glycerol operon. The resulting strain was efficiently counter-selected on glycerol-containing medium but the mutation had no effect on the capacity of the strain to be maintained in non-sterile soil samples and to transfer derivatives of the genetic element pSAM2. However, despite the efficiency of the donor counterselection system, the transfer of a pSAM2 derivative from S. lividans to indigenous soil bacteria was not detected. ß

No abstract