A gelatin gel sheet was UV-irradiated to raise the gel melting temperature T m . Three second of T m increased proportionally to irradiation time t UV . The sheet of which T m was larger than the culture temperature 37°C was used as a cell culture scaffold. Human fibroblast cells WI-38 adhered to the sheet and dug deeply into it regardless of t UV , while cancer cells WI-38-VA-13 formed spheroids and did not adhere to the sheet individually at high UV dose. The cell selectivity of the scaffold could be used for biological and medical studies.