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We have made significant progress in developing innovative methods for investigating the mechanism and extent of in situ bioremediation of chlorinated organic solvents. These methods use precise isotopic ratio measurements of chlorine and carbon in reactant and product species in laboratory experiments and in materials from field demonstration sites. Specific tasks completed during FY 1997 include: (1) refinement and publication of our new analytical method for precise determination of chlorine and carbon isotope ratios in chlorinated volatile organic compounds; (2) laboratory experiments involving biological degradation of chlorinated solvents in liquid cultures and soil columns; and (3) use of chlorine and carbon isotope ratios to investigate natural attenuation of trichloroethene at the Paducah Gaseous Diffusion Plant. This work can have immediate impact because it will provide the fundamental basis for a new and cost-effective means of evaluating and monitoring the effectiveness of in situ bioremediation schemes for chlorinated organic solvents in soils, vadose horizons, and groundwater plumes.

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