LIE, T. A., MUILENBURG, M., HIEP, N. H., and AYHAN, K. 1992. Cultivation of Bradyrhizobiurn CB756 on sucrose prefermented by yeast. Can. J. Microbiol. 38: 569-572. Saccharornyces cerevisiae, grown with sucrose as a substrate, converts the sucrose to glycerol and produces yeast cells. The yield of glycerol was doubled by adding CaCO,. The fermented medium, containing glycerol and yeast cells, was an excellent medium for the cultivation of Bradyrhizobiurn CB756. Best results were obtained after dilution of the fermented ,medium to 10% (v/v) with a basal salts solution, but even with dilutions of 5 and 2.5%, high numbers of Bradyrhizobiurn CB756 cells were obtained.

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