Opak River watershed is one of the river watershed in special region of Yogyakarta Province which has important role as water reserve for the community. The eruption of Merapi volcano that often occurs has damaged riparian ecosystem of the rivers in Opak river watershed. To restore Ecosystems in Opak river watershed Conservation during 2016-2018. Tree planting have been conducted five times in riparian ecosystem and Mangrove ecosystem during 2016-2018, At least 17000 individuals have been planted in tree area comprising ( 5000 individual of 25 species in upstream, 2000 individuals of six species and 10000 individuals of Rhizophora spp. Biodiversity survey have been conducted two time in July-August 2017 and April 2018 at three area in Opak river watershed (up stream, middle stream and down stream). The results shows a total of 291 species of plants, 84 birds species, 18 species of herpetofauna, 10 species of mammals, 35 species of fish, 206 species of insects, 26 species of macrozoobentos (mollusca), and 113 species of plankton in three study areas has been identified in Opak Watershed during 2017-2018. The presence of species in each study area as well as the composition of the number of individuals per species is shows difference between the survey in 2017 and 2018 .This is also showed in the number of those diversity indexes. This Biodiversity monitoring is planned to be continued least 5 times which will be conducted in every years.