No abstract

In the present study, a total of four species of biodegrading bacteria namely Bacillus subtilis, Eschericia coli, Staphylococcus aereus and Pseudomonas putida were isolated from soil samples collected from the sites where lead and mercury were discharged. The effect of various physicochemical parameters on growth of these bacteria was also studied.

Alfa- Feto- protein (AFP) is a protein produced by the baby in the womb. The protein is normally found in the blood stream of the mother, which tends to be elevated or lowered in the blood of the women carrying babies with certain abnormalities it is an Albumin like glycoprotein with a molecular weight of 70,000 D. Is a formed in the yolk sac. Non differential liver cells and the fetal gastro- intestinal tract. Elevated MSAFP levels have also been associated with Chromosome abnormalities, Omphalocele and gastroschisis and rarely congenital, additionally elevated MS AFP levels have associated with an increased risk for adverse perinatal out comes including preeclampsia, premature labor, low birth weight, placental abruption and stillbirth. Int. J. Appl. Sci. Biotechnol. Vol 8(2): 231-234

In the present study, a total of four species of biodegrading bacteria namely Bacillus subtilis, Eschericia coli, Staphylococcus aereus and Pseudomonas putida were isolated from soil samples collected from the sites where lead and mercury were discharged. 16s RNA is amplified using Polymerase Chain Reaction and the amplified product was confirmed by agarose gel electrophoresis and it has been subjected to sequencing and the sequence obtained was compared with the sequence obtained from the nucleotide database of NCBI