<p>A study was conducted to develop a vine multiplication package for generation of seed yam. The parameters assessed for the package were rooting media of the vine cuttings, yam variety and nutrient supplementation to the mother plant to generate minitubers. Nine varieties of yams were planted in 4 media types (soil, soil + sawdust, soil + carbonized rice husk and cocopeat) and 2 levels of nutrient supplementation (no supplementation and supplementation) using RCBD with 3 replications. The study was conducted over 2-year period. Boosting the nutrient status of the mother plant before excising the vines proved to enhance establishment and subsequently the yield of the vine cuttings. Incorporation of biochar in the planting media also enhanced establishment of the vine cuttings and subsequent yields. Success of the vine multiplication techniques also proved to be variety dependent. An in vivo vine multiplication package has been developed for yam seed generation.</p>