This paper summarizes analytical techniques in order to get a clear picture of the ins and outs of the (bio)analysis of platinum-containing compounds. The antitumour agent cisplatin has become an indispensable drug for the cure of a variety of cancer diseases. Since its introduction in the early seventies, about 2,000 related platinum complexes were designed to devoid the dose-limiting nephrotoxicity. Some of them were introduced for clinical trial, such as carboplatin and iproplatin. To investigate the mechanism of action and pharmaco-kinetic behaviour, several interesting assays for total and specific platinum determination in biological matrices have been developed, each with its own possibilities and limitations.

Aescoket | is an injection containing I[.54% (w/v) Ketamine Hydrochlorlde and 0.01% (w/v) Benzethonium Chloride in water. Both compounds could be analyzed by an HPLC system consisting of a silicagel column, dimensions 0.26 x 25 cm, particle size |0 ~m, a mobile phase of tetrabutylammonium bromide 0.[ M + dlsodlum hydrogen citrate 0.0[ M + water + methanol (i0 + 50 + 120 + 320, all by volume). Analysis of Ketamine was performed without sample clean-up; detection was performed at 219 nm. Results were compared with an assay according to Ketamine Hydrochloride injection USP XXI; the results correlated well. Benzethonium was extracted as an ion-pair with chloride into chloroform. After evaporation of the chloroform layer, the residue was redissolved in mobile phase and injected. Detection was performed at 225 nm. A stability study indicated an expiration date of two years after production by storage at room temperature.