The degree and rate of primary and secondary cycle development by Plasmodiophora brassicae in the root hairs and root medullary rays, respectively, of a completely resistant, a paritially resistant and a susceptible radish {Raphanus sativus) cultivar were compared. Seeds were germinated in an infested soil mixture and seedlings were harvested 2, 4, 6 and 8 days after sowing seed for observations of the primary cycle and 6, 12, 18, 24, 30 and 36 days after sowing seed for observations of the secondary cycle. No differences in the degree or rate of primary phase colonization were detected among the three cultivars. Initially, uni-and/or bi-nucleate secondary plasmodial stages were found in the cortex of all three cultivars. The number of these plasmodia was greatest in resistant and least in susceptible cultivars. No further colonization or symptom expression occurred in the completely resistant cultivar. In the partially resistant cultivar the appearance of advanced stage-s of the fungus secondary cycle development were delayed until day 30 as compared with colonization of the susceptible cultivar in which advanced stages appeared by day 18. k is concluded that resistance is expressed during the initial stages of secondary cycle colonization.
ZusammenfassungQuantitative Besdireibung der Kolonisierung von empfindlichen und resistenten Radiesdienpflanzen durch Plasmodiophora brassicae Der Grad und die Starke der primaren und sekundaren Entwicklungsstufe von Plasmodiophora brassicae in Wurzelhaaren und Wurzelmarkstrahlen wurde bei vollig resistenten, partiell resistenten und empfindlidien Radiesdiensorten {Rhaphanus sativus) verglichen. Die .S. Copyright Clearance Center Code Statement: 0031-9481/83/0802-0097S02.50/0 r 98 KROLL, LACY and MOORE Samcn liofcn im infizierten Boden auf. Die erste Entwicklung.sstufe wurde 2, 4, 6 und 8 Tage und die zwcite 6, 12, 18, 24, 30 und 36 Tage nadi der Saat an den geernteten Samlingen beobaditet. Kcinc Unterschicdc des Grades oder der Stiirke der ersten Kolonisationsphase wurden bei den drei Sorten beobachtet. Anfanglich wurden uni-und/oder bi-nukleare sekundare Plasmodien-Stufen in der Innenrinde aller drci Sorten gefunden. Die Zahl dieser Plasmodien war bei den resistenten Sorten am groiken und bei den empiindlichen Sorten am niedrigstcn. Keine weitere Kolonisierung oder Symptomenauspragung fand bei der vollig resistenten Sorte sta^tt. Bei der partiell resistenten Sorte wurde die Erscheinung der fortgeschrlttenen sekundaren Entwicklungsstufe des Pilzes bis auf den 30. Tag verzogert im Vergleich zu der empfindlichen Sorte, die schon nm 18. Tag diese Stufe erreichte. Es wird gefolgert, dal^ die Resisrenz wahrend der anfanglichen Phaic der sekundaren Kolonisierungsstufe ausgepragt ist.
Clubroot-susceptible ‘Scarlet Knight’ radish plants grown with resistant ‘Saxafire’ radish plants, in a Plasmodiophora brassicae Wor.-infested growth medium, had significantly lower incidence of colonization and club formation than susceptible plants grown alone. This decrease in disease development was due to a reduction in the rate and, consequently, the amount of colonization of the root cortex, medullary rays, and stele. A similar response occurred whether a susceptible plant was grown in the presence of 2, 4, or 7 resistant plants.
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