Currently, the status of water quality in the river area tends to decline where one of the causes is the land use along the Sekampung River, and the land use is dominated by agriculture. Mapping of water quality in rivers aims to provide information in the form of distribution of water quality status in rivers. The purpose of this study is to identify the type of land use in the Sekampung River, Sumur Kucing Village, analyze the water quality of the Sekampung River, Sumur Kucing Village based on physical parameters (Temperature and TDS) and chemical (pH, Nitrate, Nitrite, and Ammonia) and interpreted the results of river water quality status using the STORET method spatially thematically. Determination of sample points was carried out using purposive sampling method with 4 sample points which were representative of each land use. Sampling of river water was carried out by referring to SNI 03-7016-2004. The results show that the land use in the Sekampung River is in the form of agricultural areas, plantations, settlements, ponds, and rice fields. The results of the analysis of water quality are based on physical parameters and chemical parameters, parameters that exceed the quality standards include TDS, Ammonia, and Nitrite. The results of the water quality distribution map by determining the status of water quality using the STORET method show point 1 and point 4 are classified as lightly polluted and point 3 and point 2 are classified as moderately polluted.