G,Q and R-binding pattern comparisons between the Murrah type of Bubalus bubalis L. (2n - 50) and the Holstein Friesian breed of Bos taurus L. (2n = 60) chromosomes revealed that the autosomes are similar in both species; in the former, the five pairs of submetacentrics correspond to centric fusions of chromosomes 1-29, 2-22, 8-19, 5-28 and 16-25 in the latter. Silver staining on somatic cells of buffalo revealed telomeric AG-NORs located on six pairs of autosomes, identified as 3p, 4p, 8, 21, 23 and 24. Only one pair of nucleolus organizer chromosomes is common to both species, namely the 4p of buffalo which corresponds to the 28 of cattle. The remaining NORs are located on different pairs. Out of 18 individuals, the number of Ag-NORs per cell varied between 3 and 10, with a mean value of 6.3 +/- 1.7; the mean number of nucleolar associations per cell was between 0.1 and 1.8. In the total population of 317 cells the two variables followed the binomial and the Poisson distributions, respectively, and their correlation coefficient was not statistically significant (r = +0.36). In addition to the mean number of Ag-NORs, the mean number of nucleolar associations per cell should be used for a better understanding of the differences among individuals.

No abstract

SUMMARYThe serum of an Italian Fviesian bull (Rocket-Ab) contained autoantibodies specific for a serum antigen (R Ag ) whose expression seems age-influenced. The A precipitin line appeared between the sera of Rocket-Ab (a bull) and 1035 (a calf). At the time samples were collected, the former was five years and the latter three months old. To distinguish which of these two sera contained the antibodies, both were tested against a panel of fourty normal sera. The calf 1035 showed no reaction, whereas Rocket-Ab reacted with three animals.Obviously the latter was the source of antibodies. Rocket-Ab, in

Single radial diffusion combined with the Rose Bengal test permitted rapid identification of all of the Brucella abortus -infected cattle in a study group of 689 animals.

Summary In water buffalo, two paternal half-sibs have been identified which, when used as recipients, produced antibodies of the same specificity as those they stimulated when used as donors. The apparent serologic paradox is presumably due to an antigenic determinant located beneath the surface of erythrocytes.